Failing to establish a handshake to aws server via Clouldflare managed subdomin

Our comapany domain biokeyper.com has an ssl certificate which even covers all subdomains, we have a subdomain which points to the AWS server where the app is running, with all configurations done, trying to make HTTPS request throws Cloudflare error code 525. trying to install a new certificate on the server for the mentioned subdomain does not succeed with status code 404. making HTTP request to subdomain however is successful. Kindly help troubleshoot that issue. a guide would be helpful because our service is down and users are unable to access the it.

Your registrar is set with both AWS and Cloudflare nameservers. If you are using Cloudflare then you must have only the 2 Cloudflare nameservers entered…
https://cf.sjr.org.uk/tools/check?a250bb7f0a39473f8ed944fa0c08c764#whois

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