DNS queries to timing out

I have been managing a network and it appears something has recently begun blocking access to cloudflare dns servers and family. The url is working but dns queries fail with timeout.

The issue seems to be limited to the windows based desktop computers, as i can perform queries on the linux based routers.

Dns queries to on the other hand work fine, so it seems something is specifically blocking / / / on port 53

It seems unlikely that this is actually a cloudflare issue but since it seems to be a very specific issue i was hoping somebody might have a hint or other helpful information.